Employs both synthetic peptides and novel detergents to block the non-specific binding of proteins to nitrocellulose or to charged nylon membranes, thus reducing background levels. An indicator dye is provided to help monitor wash steps and is suitable for chemiluminescent reagents. Ten milliliters of MegaBloc 1 dilutes in water to make oneo liter of blocking solution.Blocking Reagent (Western and ELISA blots)